Algemeen

Neonicotinoid insecticides have come under scrutiny for their potential unintended effects on non-target organisms, particularly pollinators in agro-ecosystems. As part of a larger study of neonicotinoid residues associated with maize (corn) production, 76 water samples within or around the perimeter of 18 commercial maize fields and neighbouring apiaries were collected in 5 maize-producing counties of southwestern Ontario. Residues of clothianidin (mean = 2.28, max. = 43.60 ng/mL) and thiamethoxam (mean = 1.12, max. = 16.50 ng/mL) were detected in 100 and 98.7%of the water samples tested, respectively. The concentration of total neonicotinoid residues in water within maize fields increased six-fold during the first five weeks after planting, and returned to pre-plant levels seven weeks after planting. However, concentrations in water sampled from outside the fields were similar throughout the sampling period. Soil samples from the top 5 cm of the soil profile were also collected in these fields before and immediately following planting. The mean total neonicotinoid residue was 4.02 (range 0.07 to 20.30) ng/g, for samples taken before planting, and 9.94 (range 0.53 to 38.98) ng/g, for those taken immediately after planting. Two soil samples collected from within an conservation area contained detectable (0.03 and 0.11 ng/g) concentrations of clothianidin. Of three drifted snow samples taken, the drift stratum containing the most wind-scoured soil had 0.16 and 0.20 ng/mL mainly clothianidin in the melted snow. The concentration was at the limit of detection (0.02 ng/mL) taken across the entire vertical profile. Our results suggest that neonicotinoids may move off-target by wind erosion of contaminated soil. These results are informative to risk assessment models for other non-target species in maize agro-ecosytems

Earthworms (Eisenia fetida) are one of the most abundant terrestrial species, and play an important role in maintaining the ecological function of soil. Neonicotinoids are some of the most widely used insecticides applied to crops. Studies on the effect of neonicotinoids on E. fetida are limited. In the present work, we evaluated the effects of five neonicotinoid insecticides on reproduction, cellulase activity and the tissues of E. fetida. The results showed that, the LC50 of imidacloprid, acetamiprid, nitenpyram, clothianidin and thiacloprid was 3.05, 2.69, 4.34, 0.93 and 2.68 mg kg−1, respectively. They also could seriously affect the reproduction of E. fetida, reducing the fecundity by 84.0%, 39.5%, 54.3%, 45.7% and 39.5% at the sub-lethal concentrations of 2.0, 1.5, 0.80, 2.0 and 1.5 mg kg−1, respectively. The cellulase activity of E. fetida was most sensitive to clothianidin. Significant disruption of the epidermal and midgut tissue was observed after 14 d exposure. In summary, we demonstrate that imidacloprid, acetamiprid, nitenpyram, clothianidin and thiacloprid have high toxicity to earthworm, and can significantly inhibit fecundity and cellulase activity of E. fetida, and they also damage the epidermal and midgut cells of earthworm.

Warum verlieren wir jedes Jahr weltweit 24 Milliarden Tonnen fruchtbaren Boden? Welche Landwirtschaft brauchen wir, um unsere Böden langfristig gesund zu erhalten? Um solche Fragen zu diskutieren, treffen sich vom 19. bis zum 23. April 2015 mehr als 450 Teilnehmer aus Politik Forschung und Praxis auf der 3. Global Soil Week in Berlin. Dazu passend veröffentlichte das Umweltbundesamt (UBA) jüngst einen aufrüttelnden Hintergrundbericht zur Landwirtschaft in Deutschland - und deren Folgen für die Böden. Der Tenor: Stickstoff, Phosphor, Schwermetalle - unsere Landwirte holen zwar das Mögliche aus den Böden heraus. Aber sie tun es nicht nachhaltig. Der übermäßige Einsatz von Mineraldüngern, Gülle und Pestiziden macht die Böden auf Dauer krank. Und schädigt auch das Grundwasser, das Klima und die Artenvielfalt auf den Feldern.

An influential European scientific body said on Wednesday that a group of pesticides believed to contribute to mass deaths of honeybees is probably more damaging to ecosystems than previously thought and questioned whether the substances had a place in sustainable agriculture. The finding could have repercussions on both sides of the Atlantic for the companies that produce the chemicals, which are known as neonicotinoids because of their chemical similarity to nicotine. Global sales of the chemicals reach into the billions of dollars. Research has been directed largely at the effects of neonicotinoids on honeybees, but that focus “has distorted the debate,” according to the report released on Wednesday by the European Academies Science Advisory Council. The council is an independent body composed of representatives from the national science academies of European Union member states. The European ban is up for review this year, and the council’s report, based on the examination of more than 100 peer-reviewed papers that were published since the food safety agency’s finding, was prepared to provide officials with recommendations on how to proceed.

The objective of this study was to assess whether changes in the structure of the soil microbial community after imidacloprid application at the field rate (FR, 1 mg/kg soil) and 10 times the FR (10 × FR, 10 mg/kg soil) may also have an impact on biochemical and microbial soil functioning. The obtained data showed a negative effect by imidacloprid applied at the FR dosage for substrate-induced respiration (SIR), the number of total bacteria, dehydrogenase (DHA), both phosphatases (PHOS-H and PHOS-OH), and urease (URE) at the beginning of the experiment. In 10 × FR treated soil, decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over the experimental period. Nitrifying and N2-fixing bacteria were the most sensitive to imidacloprid. The concentration of NO3− decreased in both imidacloprid-treated soils, whereas the concentration of NH4+ in soil with 10 × FR was higher than in the control. Analysis of the bacterial growth strategy revealed that imidacloprid affected the r- or K-type bacterial classes as indicated also by the decreased eco-physiological (EP) index. Imidacloprid affected the physiological state of culturable bacteria and caused a reduction in the rate of colony formation as well as a prolonged time for growth. Principal component analysis showed that imidacloprid application significantly shifted the measured parameters, and the application of imidacloprid may pose a potential risk to the biochemical and microbial activity of soils.

Neonicotinoids are the most widely used insecticides world-wide, but their fate in the environment remains unclear, as does their potential to influence non-target species and the roles they play in agroecosystems. We investigated in laboratory and field studies the influence of the neonicotinoid thiamethoxam, applied as a coating to soya bean seeds, on interactions among soya beans, nontarget molluscan herbivores and their insect predators. In the laboratory, the pest slug Deroceras reticulatum was unaffected by thiamethoxam, but transmitted the toxin to predaceous beetles (Chlaenius tricolor), impairing or killing >60%. In the field, thiamethoxam-based seed treatments depressed activity–density of arthropod predators, thereby relaxing predation of slugs and reducing soya bean densities by 19% and yield by 5%. Neonicotinoid residue analyses revealed that insecticide concentrations declined through the food chain, but levels in field-collected slugs (up to 500 ng g1) were still high enough to harm insect predators. Our findings reveal a previously unconsidered ecological pathway through which neonicotinoid use can unintentionally reduce biological control and crop yield. Trophic transfer of neonicotinoids challenges the notion that seed-applied toxins precisely target herbivorous pests and highlights the need to consider predatory arthropods and soil communities in neonicotinoid risk assessment and stewardship.

Imidacloprid is one of the most commonly used insecticides in agricultural practice, and its application poses a potential risk for soil microorganisms. The objective of this study was to assess whether changes in the structure of the soil microbial community after imidacloprid application at the field rate (FR, 1 mg/kg soil) and 10 times the FR (10 × FR, 10 mg/kg soil) may also have an impact on biochemical and microbial soil functioning. The obtained data showed a negative effect by imidacloprid applied at the FR dosage for substrate-induced respiration (SIR), the number of total bacteria, dehydrogenase (DHA), both phosphatases (PHOS-H and PHOS-OH), and urease (URE) at the beginning of the experiment. In 10 × FR treated soil, decreased activity of SIR, DHA, PHOS-OH and PHOS-H was observed over the experimental period. Nitrifying and N2-fixing bacteria were the most sensitive to imidacloprid. The concentration of NO3− decreased in both imidacloprid-treated soils, whereas the concentration of NH4+ in soil with 10 × FR was higher than in the control. Analysis of the bacterial growth strategy revealed that imidacloprid affected the r- or K-type bacterial classes as indicated also by the decreased eco-physiological (EP) index. Imidacloprid affected the physiological state of culturable bacteria and caused a reduction in the rate of colony formation as well as a prolonged time for growth. Principal component analysis showed that imidacloprid application significantly shifted the measured parameters, and the application of imidacloprid may pose a potential risk to the biochemical and microbial activity of soils.

In this research study, time-dependent sorption behavior of Dinetofuran (DNT), imidacloprid (IMD) and thiamethoxam (THM) in vineyard soil was studied. Sorption kinetics studies were conducted over a period of 96 hours with sampling duration varying from 0, 2, 4, 8, 12, 24, 60 and 96 hours. All three neonicotinoids exhibited very low sorption potential for the soil investigated. Overall percent sorption for all three neonicotinoids was below 20.04 ± 2.03% with highest percent sorption being observed for IMD followed by DNT and THM. All three neonicotinoids are highly soluble with solubility increasing with IMD < THM < DNT. Although, DNT has the highest solubility among all three neonicotinoids investigated, it exhibited higher percent sorption compared to THM, indicating factors other than solubility influenced the sorption kinetics. Low sorption potential of neonicotinoids indicates greater leaching potential with regard to groundwater and surface water contamination.

Earthworms provide key soil functions that favour many positive ecosystem services. These services are important for agroecosystem sustainability but can be degraded by intensive agricultural practices such as use of pesticides. Many literature reports have investigated the effect of pesticides on earthworms. Here, we review those reports to assess the relevance of the indicators of earthworm response to pesticides, to assess their sensitivity to pesti- cides, and to highlight the remaining knowledge gaps. We focus on European earthworm species and products authorised in Europe, excluding natural compounds and metals. We consider different organisation levels: the infra-individual level (gene expression and physiology), the individual and population levels (life-history traits, population density and behaviour) and the community level: community biomass and density. Our analysis shows that earthworms are impacted by pesticides at all organisation levels. For example, pesticides disrupt enzymatic activities, increase individual mortality, decrease fecundity and growth, change individual behaviour such as feeding rate and decrease the overall community biomass and density.

The metabolic degradation and persistence of imidacloprid in paddy field soil were investigated following two applications of imidacloprid at 20 and 80 g a.i. ha−1 at an interval of 10 days. The soil samples were collected at various time intervals. The limit of quantification for the analysis of imidacloprid and its metabolites was obtained at the concentration of 0.01 mg kg−1. The initial deposits of total imidacloprid were found to be 0.44 and 1.61 mg kg−1 following second applications. These residues could not be detected after 60 and 90 days following second applications of imidacloprid at lower and higher dosages, respectively. In soil, urea metabolite was found to be the maximum, followed by olefine, nitrosimine, 6-chloronicotinic acid, 5-hydroxy and nitroguanidine. The half-life values (t 1/2) of imidacloprid were worked out to be 12.04 and 11.14 days, respectively, when applied at lower and higher doses, respectively.