A subacute toxicity study of acetamiprid was undertaken in 72 female wistar rats in four groups (18 each). Three different concentrations of acetamiprid (25, 100 and 200 mg/kg of body weight) were administered orally to rats. Untreated rat served as control. The antibody titre of sheep red blood cells (SRBC) in all the treated groups and decrease in cell mediated immune response (CMI) were evaluated by delayed type hypersensitivity reaction to dinitroflurobenzene (DNFB). Our results revealed that in 200 mg/kg body treated rats, there were significant (Pd≤0.01) decrease in mean values of total leukocyte count and relative lymphocyte count in rats. Globulin was also decreased significantly (Pd≤0.01) in acetamiprid treated rats compared to control. There was a significant decrease (Pd≤0.01) in spleen weight in rats treated with 200 mg/kg dose as compared to control. Histopathological examination of spleen revealed depletion of lymphocytes from Malpighian corpuscles in all treated groups in a dose dependent manner. The results indicated that acetamiprid suppressed both CMI and antibody forming ability of lymphocytes.
Source:Mondal S et al Proceedings of the Zoological Society
Volume 62, Number 2, 109-117, DOI: 10.1007/s12595-009-0012-6
http://www.springerlink.com/content/a72471w669p10533/
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